During glycolysis, which enzyme is responsible for generating ATP?

Biochemistry MCQs for PPSC, FPSC, NTS, and Pakistan government job tests. Select an option below, then read the explanation.

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Subject
Biochemistrymedical-mcqs › biochemistry
Published
15 Jul 2019
Last updated
28 May 2026

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Explanation

Phosphoglycerate kinase catalyzes a substrate-level phosphorylation step in glycolysis, producing ATP. Hexokinase and phosphohexose isomerase are involved in earlier steps without ATP generation, while enolase catalyzes the formation of phosphoenolpyruvate and does not directly produce ATP.

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