According to the Michaelis-Menten theory, what is a fundamental aspect of enzymatic reactions?

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The speed of an enzyme-catalyzed reaction does not depend on substrate levels.Non-enzymatic reaction rates increase in direct proportion to substrate amount.Km represents the binding affinity constant between enzyme and substrate.The creation of an enzyme-substrate complex is crucial for enzymatic activity.

Explanation

The Michaelis-Menten hypothesis emphasizes that the formation of an enzyme-substrate complex is a necessary step in enzymatic reactions. This complex formation allows the enzyme to convert substrate into product. Other options are incorrect because enzymatic reaction rates generally depend on substrate concentration, Km is a constant related to enzyme kinetics but not simply the association constant, and non-enzymatic reaction rates do not follow the same principles.