Protein denaturation occurs due to changes in which level(s) of its structure?

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The amino acid sequence (primary structure)The local folding patterns like alpha-helices and beta-sheets (secondary structure)The overall 3D conformation (tertiary structure)Both the secondary and tertiary structuresNone of the above

Explanation

Protein denaturation involves the disruption of the protein's secondary and tertiary structures, which affect its folding and 3D shape. The primary structure, or amino acid sequence, remains unchanged during denaturation.