The Lancefield grouping system for beta-hemolytic streptococci is determined by which of the following?

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M protein structurePolysaccharide C antigen compositionLipid types present in the cell membranePhysiological characteristicsCapsular polysaccharides

Explanation

Lancefield classification of beta-hemolytic streptococci is based on the specific polysaccharide C antigen found on the bacterial cell wall, which differentiates the groups. Other factors like M protein, membrane lipids, or physiological traits are not used for this classification.